The study investigates the influence of syngas composition, primarily CO, CO2, and H2, on the production of ethanol and other valuable compounds by Clostridia bacteria. Notably, C. carboxidivorans demonstrated the highest ethanol yield when exposed to a gas mixture high in CO content. The research also delved into the production of various C2-C6 compounds like lactate, propionate, butyrate, and more through the Wood-Ljungdahl pathway. Models like the Modified Gompertz model were used effectively to describe cell growth and ethanol production kinetics. The significance of optimizing syngas composition for maximal ethanol and biochemical production was highlighted, offering a sustainable approach to biofuels and bioproducts. With a focus on renewable energy development to meet EU emission reduction goals and enhance energy frameworks, syngas, a versatile gas mixture, offers the potential to produce valuable chemicals efficiently. The study underscores the advantages of syngas fermentation over conventional methods, emphasizing the role of specific Clostridia strains in bioconversion processes. While challenges like syngas composition variability persist, the research sheds light on the need for tailored gas mixtures to enhance metabolite yields. Medium-chain acids and alcohols are proposed as alternatives to conventional biofuels, given their higher energy density and compatibility with fuel infrastructure. The study encourages further exploration of syngas blends to optimize product yields beyond ethanol, paving the way for a more diverse and efficient biofuel production landscape.