The increasing global demand for energy, coupled with the adverse environmental impacts of fossil fuels, has led to a growing focus on alternative, sustainable energy sources. Hydrogen gas, recognized for its carbon-free properties, is positioned to address a substantial portion of the world's energy needs by 2050. Biohydrogen, a form of hydrogen derived from biological sources, offers a clean and environmentally friendly energy solution with the potential to contribute significantly to global energy consumption. The versatility and efficiency of biohydrogen highlight its role in power generation while minimizing environmental harm. Regions like California, Europe, Japan, and Germany are making significant strides in developing hydrogen infrastructure to support the integration of hydrogen as an energy source. Notably, microorganisms, especially microalgae, are being extensively studied for their ability to produce biohydrogen through processes such as photo-fermentation and dark-fermentation. These research efforts underscore the promising future of biohydrogen as a sustainable energy option, showcasing the ongoing advancements in harnessing renewable resources for a greener tomorrow.