Algae have emerged as a promising source for hydrogen production, a green energy carrier. Researchers at Ruhr University Bochum, Germany, delved into the mechanisms behind this phenomenon, particularly focusing on the enzyme HydF and its role in assembling the hydrogen cluster within unicellular algae. The study highlighted the importance of the [FeS] clusters in catalyzing hydrogen production and how specific ligands on the cluster enable this process. Through site-specific mutagenesis, the researchers explored how individual amino acids contribute to the synthesis and anchoring of the cofactor precursor. Their findings provide a deeper understanding of HydF's function during the synthesis of essential ligands for hydrogen turnover. By utilizing techniques like hydrogen production measurements and spectroscopy, the team elucidated the intricate steps involved in the biosynthesis of the cofactor within [FeFe]-hydrogenases. This research not only uncovers the mysteries surrounding algae's hydrogen production but also offers insights that could propel advancements in sustainable energy production and the development of novel biotechnological applications.