The U.S. Department of Energy's Bioenergy Technologies Office (BETO) recently announced the release of an important Request for Information (RFI) focused on advancing net-zero emission propulsion technologies for the off-road transportation sector. This RFI, conducted jointly with the Vehicle Technologies Office (VTO) and Hydrogen and Fuel Cell Technologies Office (HFTO), seeks to gather valuable insights into the preferences for alternative propulsion technologies in the off-road sector, identify promising technologies, and pinpoint key barriers hindering the transition to net-zero emissions by 2050. Given that the off-road transportation sector contributes ten percent of U.S. transportation-related greenhouse gas emissions, the RFI's primary objective is to leverage stakeholder knowledge to inform decisions on future propulsion technologies, research directions, infrastructure investments, and coordination efforts to ensure the sector aligns with or surpasses U.S. decarbonization targets. To achieve this, the RFI poses critical questions about the current status of the off-road vehicle fleet, the most viable powertrain technologies for decarbonization, and the timeline for transitioning to net-zero emission technologies. Various propulsion technologies are being considered in the RFI, including biodiesel, renewable diesel, renewable natural gas, battery electric options, direct electrification, electric hybridization, hydrogen-fueled internal combustion engines, and hydrogen fuel cells. Notably, the RFI highlights that no single propulsion technology currently stands out as the clear choice for decarbonizing the off-road sector, underscoring the need for further insights and innovation. Interested parties are invited to submit their responses in Microsoft Word format to CleanMachines@ee.doe.gov by June 7, 2024, to contribute to shaping the national strategy for decarbonizing the off-road transportation sector. This collaborative effort signifies a positive step towards addressing emissions in the off-road industry and fostering sustainable propulsion solutions for the future.