Hydrogen has emerged as a clean and efficient energy carrier with the potential to replace fossil fuels, gaining global attention for its environmental benefits. Biohydrogen production, particularly from coffee wastewater, has been highlighted as a sustainable alternative to conventional methods. The study conducted in Iran focused on utilizing 800 m3/day of coffee wastewater with high carbohydrate content for biohydrogen production. The research integrated Techno-Economic Analysis (TEA) and Life Cycle Analysis (LCA) to assess the economic feasibility and environmental impact of the biohydrogen production process. By simulating the mass and energy balance of the process using Aspen Plus software and conducting LCA with SimaPro, the study aimed to provide valuable insights into the potential of biohydrogen production from coffee wastewater. The research also explored the use of pilot plants to scale up biohydrogen production, showcasing the possibility of utilizing waste materials for renewable energy generation. Comparisons between thermophilic and mesophilic conditions further enriched the study by evaluating different aspects such as mass, energy, economy, environmental effects, and the quantity of biohydrogen and by-products generated. The findings of this study contribute to the ongoing efforts in the field of renewable energy and highlight the significance of exploring sustainable alternatives for hydrogen production.