Horizon Europe's Cities Mission, launched in 2021, has outlined its priorities for the coming years, focusing on fostering sustainable and climate-neutral cities. The mission aims to transform 100 selected cities into innovation hubs that will set an example for urban centers globally by 2050. The draft work programme reveals ten key areas of interest, including circular economy models in construction, low-voltage direct current microgrids, low-temperature heating systems, hydrogen valleys, urban wastewater management, nature restoration, multimodal passenger hubs, energy-efficient public transport, waste reduction in e-commerce, and innovative procurement. Notably, the mission emphasizes collaboration with programs like the Clean Hydrogen joint undertaking to promote hydrogen energy solutions for decarbonization. By encouraging the adoption of innovative technologies and sustainable practices, Horizon Europe's Cities Mission seeks to drive positive environmental and societal impact for urban communities.