Energy is essential for global economic development, with the shift towards renewable sources like hydrogen gaining momentum due to environmental concerns. Recent research focuses on utilizing hydrogen, particularly in transportation, with sodium borohydride (NaBH4) emerging as a promising source. The methanolysis of NaBH4 for hydrogen production shows potential, with the need for effective catalysts. Various catalysts, including metal nanoparticles and bio-support materials like Bacillus atrophaeus bacteria, are being explored for SB methanolysis. Bacillus atrophaeus, a bacteria known for its agricultural and sterilization benefits, is being tested as a bio-supporter for copper nanoparticles in hydrogen production. Preliminary tests have indicated its suitability for SB solvolysis due to its solubility in alcohol and eco-friendly properties. This study aims to develop a unique copper nanoparticle using Bacillus atrophaeus as a support material, potentially revolutionizing the field of hydrogen production by offering a cost-effective and green approach. By grafting copper nanoparticles on Bacillus atrophaeus during SB methanolysis, researchers aim to discover new possibilities for sustainable hydrogen generation.