Hydrogen, as a clean and high-energy-density fuel, is gaining traction as a promising alternative to fossil fuels for a sustainable energy future. One crucial aspect of hydrogen production is the steam reforming of bio-ethanol (SRE), which offers a green pathway for hydrogen generation. While nickel has been a standard metal catalyst for SRE due to its efficiency in bond dissociation and cost-effectiveness, challenges like deactivation through sintering and carbon deposition have persisted. To address these limitations, recent research has focused on innovative catalyst designs, notably single-atom catalysts (SACs) and their synergistic combination with nanoclusters. The article discusses a study that successfully synthesized a series of xNi/Mo2TiAlC2 catalysts to enhance the efficiency of ethanol reforming for hydrogen production. The study demonstrated that the 1Ni/MTAC catalyst from the series exhibited exceptional SRE activity with a hydrogen utilization efficiency of 67.8% at 550°C, remaining stable over 120 hours without deactivation. This catalyst design, incorporating a combination of single atoms and adjacent nanoclusters, showed promising results in promoting ethanol adsorption, activation, and overall catalytic performance. Notably, the hydrogen yield per Ni atom increased by 700% compared to traditional catalysts, underscoring the effectiveness of the new catalyst configuration. Characterization of the Ni/MTAC catalysts involved various analyses, including XRD patterns, SEM, TEM images, and HAADF-STEM analysis, illustrating the structural details and composition of the synthesized catalysts. The findings highlight the significance of exploring advanced catalyst designs, such as SACs and nanocluster-SAC combinations, in optimizing bio-ethanol reforming for efficient green hydrogen production. The research provides valuable insights into the potential of tailored catalyst structures for enhancing the performance and stability of hydrogen production processes.